Stephanopoulos Lab

We work on the design, synthesis, and application of functional nanomaterials using self-assembling molecules from biology.

Contact Us

727 E. Tyler St., Tempe, AZ 85287

The Biodesign Institute, Room A120B

nstepha1@asu.edu 

480-727-3443

PUBLICATIONS

Publications as an ASU faculty member

(*= corresponding author)

A. Buchberger, C.R. Simmons, N.E. Fahmi, R. Freeman, N. Stephanopoulos* "Hierarchical Assembly of DNA Origami Nanostructures Using Coiled-coil Peptides" (preprint on chemRxiv).

N. Stephanopoulos"Peptide–Oligonucleotide Hybrid Molecules for Bioactive Nanomaterials" Bioconj. Chem. (2019) 30:1915-1922.

N. Stephanopoulos* "Strategies for stabilizing DNA nanostructures to biological conditionsChemBioChem (2019) 20:2191-2197.

Y. Xu, S. Jiang, C. Simmons, R.P. Narayanan, F. Zhang, A.-M. Aziz, H. Yan, N. Stephanopoulos* “Tunable nanoscale cages from self-assembling DNA and protein building blocksACS Nano (2019) 13:3545–3554.

A. Stelson, M. Liu, C.A.E. Little, C.J. Long, N.D. Orloff, N. Stephanopoulos*, J.C. Booth* "Label-Free Detection of Conformational Changes in Switchable DNA Nanostructures with Microwave Microfluidics" Nat. Commun. (2019) 10:1174.

T. MacCulloch, A. Buchberger, N. Stephanopoulos*Emerging applications of peptide–oligonucleotide conjugates: bioactive scaffolds, self-assembling systems, and hybrid nanomaterials" Org. Biomol. Chem. (2019) 17:1668-1682

M. Liu, S. Jiang, O. Loza, P. Sulc, N.E. Fahmi, N. Stephanopoulos* “Rapid Photoactuation of a DNA Nanostructure using an Internal Photocaged Trigger StrandAngew. Chem. Int. Ed. (2018) 57:9341 –9345. (selected as paper for Wiley’s Joint Special Collection on Biopolymers, for the Murray Goodman Award Symposium at the 2019 ACS Spring Meeting: bit.ly/wileybiopolymers19)

C. Simmons, F. Zhang, T. MacCulloch, N.E. Fahmi, N. Stephanopoulos, Y. Liu, N. Seeman, H. Yan*, "Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals J. Am. Chem. Soc. (2017) 139:11254-11260.

D. Varun, G.R. Srinivaan, Y.-H. Tsai, H.-J. Kim, J. Cutts, F. Petty, R. Merkley, N. Stephanopoulos, D. Dolezalova, M. Marsala, D.A. Brafman*, “A Robust Vintronectin-Derived Peptide for the Scalable Long-term Expansion and Neuronal Differentiation of Human Pluripotent Stem Cell (hPSC)-derived Neural Progenitor Cells (hNPCs)Acta Biomater. (2017) 48:120-130.

Publications prior to ASU 

(* = co-first authors)

 

R. Freeman, M. Han, Z. Álvarez, J.A. Lewis, J.R. Wester, N. Stephanopoulos, M.T. McClendon, C. Lynsky, J.M. Godbe, H. Sangji, E. Luijten, S.I. Stupp, “Reversible self-assembly of superstructured networksScience (2018) 362:808-813.

J.J. Greene, M.T. McClendon, N. Stephanopoulos, S.I. Stupp, C.-P. Richter, “Electrophysiological assessment of a peptide amphiphile nanofiber nerve graft for facial nerve repairJ. Tissue Eng. Regen. Med. (2018) 12:1389–1401.

A.J. Matsuoka , Z.A. Sayed, N. Stephanopoulos, E.J. Berns, A.R. Wadhwani, Z.D. Morrissey, D.M. Chadly, S. Kobayashi, A.N. Edelbrock, T. Mashimo, C.A. Miller, T.L. McGuire, S.I. Stupp, J.A. Kessler “Creating a stem cell niche in the inner ear using self-assembling peptide amphiphiles PLoS ONE (2017) 12:e0190150.

R. Freeman*, N. Stephanopoulos*, Z. Álvarez, J.A. Lewis, S. Sur, C.M. Serrano, J. Boekhoven, S.S. Lee, S.I. Stupp, “Instructing cells with programmable DNA-peptide hybridsNat. Commun. (2017) 8:15982

C. Rubert-Perez, N. Stephanopoulos, S.S. Lee, S. C. Newcomb, Sur, S.I. Stupp, “The Powerful Functions of Peptide-Based Bioactive Matrices for Regenerative Medicine” (invited review) Ann. Biomed. Eng. (2015) 43:501-514.

N. Stephanopoulos, R. Freeman, H.N. Scheler, S. Sur, S. Jeong, F. Tantakitti, J.A. Kessler, S.I. Stupp, “Bioactive DNA-Peptide Nanotubes Enhance the Differentiation of Neural Stem Cells Into NeuronsNano Lett. (2015) 15:603-609.

A. Li, A. Hokugo, A. Yalom, E.J. Berns, N. Stephanopoulos, M.T. McClendon, L.A. Segovia, I. Spigelman, S.I. Stupp, R. Jarrahy., “A bioengineered peripheral nerve construct using aligned peptide amphiphile nanofibersBiomaterials (2014) 35:8780-8790.

N. Stephanopoulos, J.H. Ortony, S.I. Stupp, “Self-Assembly for the Synthesis of Functional Biomaterials” (invited review) Acta Materialia (2013) 61:912-930.

 

J. Sack, N. Stephanopoulos, D.C. Austin, M.B. Francis, J.S. Trimmer, “Antibody-guided photoablation of voltage-gated potassium channelsJ. Gen. Physiol. (2013) 142:315-324.

N. Stephanopoulos, M.B. Francis, “Choosing an Effective Protein Bioconjugation Strategy.” (invited review) Nat. Chem. Biol. (2011) 7:876-884.

 

P.G. Holder, D.T. Finley, N. Stephanopoulos, R. Walton, D.S. Clark, M.B. Francis, “Dramatic Thermal Stability of Virus-Polymer Conjugates in Hydrophobic SolventsLangmuir (2010) 26:17383–17388.

N. Stephanopoulos, G.J. Tong, S.C. Hsiao, M.B. Francis, “Dual-Surface Modified Virus Capsids for Targeted Delivery of Photodynamic Agents to Cancer CellsACS Nano (2010) 4:6014-6020.

N. Stephanopoulos*, M. Liu*, G.J. Tong, Z. Li, Y. Liu, H. Yan, M.B. Francis, “Immobilization and One-Dimensional Arrangement of Virus Capsids with Nanoscale Precision Using DNA OrigamiNano Lett. (2010) 10:2714-2720.

R.A. Miller, N. Stephanopoulos, J.M. McFarland, A.S. Rosko, P.L. Geissler, M.B. Francis, “The Impact of Assembly State on the Defect Tolerance of TMV-based Light Harvesting ArraysJ. Am. Chem. Soc. (2010) 132:6068-6074.

N. Stephanopoulos, Z.M. Carrico, M.B. Francis, “Nanoscale Integration of Sensitizing Chromophores and Porphyrins Using Bacteriophage MS2Angew. Chem. Int. Ed. (2009) 121:9662-9666.

N. Stephanopoulos, E.O.P. Solis, G. Stephanopoulos, “Nanoscale process systems engineering: Toward molecular factories, synthetic cells, and adaptive devices” (invited perspective) AIChE J. (2005) 51:1858-1869.

Invited book chapters

(*= corresponding author)

N. Stephanopoulos*, R. Freeman*, “DNA-based materials as self-assembling scaffolds for interfacing with cells”, “Self-Assembling Biomaterials: Molecular Design, Characterization and Application in Biology and Medicine, 1st Edition” 2018, pp. 157-175. (Elsevier)

L. Avolio, D. Sipes, N. Stephanopoulos, S. Sur*, “Recreating stem-cell niches using self-assembling biomaterials”, “Self-Assembling Biomaterials: Molecular Design, Characterization and Application in Biology and Medicine, 1st Edition” 2018, pp. 421-454. (Elsevier)

N. Stephanopoulos, M.B. Francis*, “Making New Materials from Viral Capsids”, “Polymer Science: A Comprehensive Reference, 1st Edition” 2012, Vol. 9, pp. 247-266. (Elsevier)